Interaction of cytochrome b5 with surfactant vesicles.
نویسندگان
چکیده
Changes in the u.v.-visible absorption spectrum of cytochrome b5 which occur upon addition of dicetyl phosphate, dimyristoyl phosphatidic acid (DMPA), or mixed DMPA/dimyristoyl phosphatidylcholine vesicles are consistent with haem transfer from the cytochrome to the membrane via an intermediate haemoprotein species. The effects of vesicle charge and concentration on the kinetics of haem transfer are discussed.
منابع مشابه
Interaction of non-myristoylated NADH-cytochrome b5 reductase with cytochrome b5-dimyristoylphosphatidylcholine vesicles.
An expression vector for NADH-cytochrome b5 reductase containing a thrombin cleavage site directly before the N-terminal glycine residue of the flavoprotein was used to isolate the non-myristoylated enzyme by thrombin cleavage of the initial fusion protein of a short segment of the multiple cloning site of the plasmid vector and the reductase. This flavoprotein preparation, containing only the ...
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ورودعنوان ژورنال:
- The Biochemical journal
دوره 251 2 شماره
صفحات -
تاریخ انتشار 1988